42) Prague Medical Report / Vol. 111 (2010) No. 1, p. 4254 parity of triad Methods for Determination of Glucose Dohnal L.1, Kalousová M.2, Zima T.2 Reference testing ground for Clinical chemistry of the Czech Ministry of Health, Prague, Czech Republic; 2 Charles University in Prague, outgrowth efficacy of Medicine and General University Hospital, Institute of Clinical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic 1 Re c e i v e d J u l y 1 3 , 2 0 0 9 ; A c c e p t e d J a n u a r y 1 9 , 2 0 1 0 . give away words: Glucose electrochemical role Spectrophotometric closing Repeatability Reproducibility par Passing-Bablok regression Traceability Abstract: analyse refers comparison of three politeness for glucose determination precision (repeatability, reproducibility), traceability to SRM 965a NIST, comparability in kindred-pools and in patients samples: Electrochemical determination on Super GL (DiaSys, Germany) in hemolyzate GL m ode, spectrophotometric determination using hexokinase (Glucose System Reagent 800, Olympus) HKL method and using glucose dehydrogenase (Glucose Gluc-DH, EcolineS+, DiaSys, Germany) GDL method in hemolyzate.
For exhibit differences between the absorption of glucose in hemolyzed blood and corresponding plasma, spectrophotometric determination using hexokinase in plasma was utilise (Glucose System Reagent 800, Olympus) HKP method. Coefficients of divergence characterizing precision under repeatability and reproducibility conditions be not last than 3.0% for the GL method, 6.3% for the GDL method and 15.8% for the HKL method with lo w sensitivity. For glucose ducking less tha! n 8 mmol/l, HKL disco biscuitds to give lower results than GDL, and GL pitchs to give higher results than GDL. For glucose concentration about 2 mmol/l, the results of glucose in plasma HKP method tend to be significantly lower (by more than ten percent) than in corresponding total (hemolyzed) blood. HKL...If you want to get a amply essay, order it on our website: BestEssayCheap.com
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